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供應(yīng)Active Rac1

[Rac

Rac1

Rac1-GTP]

武漢市Active Rac1廠家

采購數(shù)量

1 其他以上

價格

￥4800.00

運費： 湖北武漢市洪山區(qū) | 賣家支付運費

購買數(shù)量： 庫存 30000其他

在線詢盤聯(lián)系廠家：15071267082 027-87561033

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[Rac Rac1 Rac1-GTP]

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詳細(xì)信息（供應(yīng)Active Rac1）

分類： 抗體
級別： 分析純AR
含量： 1mg/ml
產(chǎn)品規(guī)格： 30μl
貨號： 80203
規(guī)格： 30μl

Active Rac1-GTP [26903]
[30 ?L]

Catalog Number: 26903

Gene Symbol: RAC1

Description: Anti-Active Rac1-GTP Mouse Monoclonal Antibody

Background: Small GTPases are a super-family of cellular signaling regulators. Rac belongs to the Rho sub-family of GTPases that regulate cell motility, cell division, and gene transcription. GTP binding increases the activity of Rac, and the hydrolysis of GTP to GDP renders it inactive. GTP hydrolysis is aided by GTPase activating proteins (GAPs), while exchange of GDP for GTP is facilitated by guanine nucleo exchange factors (GEFs).

Immunogen: Recombinant full length protein of active Rac1

Applications: IP, IHC

Recommended Dilutions: 1 ?g for 1~2 mg total cellular proteins for IP;
1:100 dilution for IHC

Conceation: 1 mg/ml

Host: mouse

Format: Liquid

Clonality: Monoclonal

Isotype: IgM

Purity: Purified from ascites

Storage buffer:

Preservative: no

Constituents: PBS (without Mg²⁺ and Ca²⁺), pH 7.4, 150 mM NaCl, 50% glycerol

Species Reactivity: Anti-active Rac1 antibody recognizes active Rac1 from vertebrates.

Storage Conditions: Store at -20°C. oid freeze / thaw cycles

Download Datasheet:

Immunoprecipitation/Western blot:

Rac activation assay. MEF cells were treated with (lane 2) or without (lane 1) PDGF. Cell lysates were incubated with an anti-active Rac monoclonal antibody (Cat # 26903) (top panel). Th precipitated active Rac was immunoblotted with an anti-Rac rit polyclonal antibody (Cat # 21003). The bottom panel shows the Western blot with anti-Rac of the cell lysates used (5% of that used in the top panel).

Immunohistochemistry:

Immunohistochemistry for the active Rac using Anti-Active Rac1-GTP Mouse Monoclonal Antibody [26903] shows Rac-GTP immunolabeling (green) in combination with cofilin (red) on brain tissue sections.
The tissue sections were fixed with -20 ?C methanol or 4% paraformaldehyde (fixation time 1hr) and stained with antibody at 1:1000 in 0.1M Phosphate buffer with 0.3% Triton X, and 4% BSA for 24h at room temperature. Secondary antibodies were anti-mouse AlexaFluor488 and anti-rit AlexaFluor594 at 1:1000.

標(biāo)簽： Rac Rac1 Rac1 Rac Rac1 Rac1-GTP 武漢市Active Rac1 武漢市Active Rac1廠家