Rac-GTP 小鼠單抗/費斯德類目 > 商鋪首頁 > 產(chǎn)品展示 > 武漢市小鼠單抗費斯德廠家_Rac GTP 活性抗體/ 抗體現(xiàn)貨/高質(zhì)量/ Rac-GTP 小鼠單抗/費斯德

Rac GTP 活性抗體/ 抗體現(xiàn)貨/高質(zhì)量/ Rac-GTP 小鼠單抗/費斯德

[Rac-GTP 小鼠單抗

Rac antibody

acitve Rac抗體]

武漢市小鼠單抗費斯德廠家

價格： 電議

物流： 湖北武漢市| 賣家支付運費

可銷售總量： 99件

手機(jī)： 18162422647 郵箱： nebio0923@163.com

傳真： 尚未完善 地址： 湖北武漢市

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[Rac-GTP 小鼠單抗 Rac antibody acitve Rac抗體]

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詳細(xì)信息（Rac GTP 活性抗體/ 抗體現(xiàn)貨/高質(zhì)量/ Rac-GTP 小鼠單抗/費斯德）

分類： 抗體
級別： 其它
產(chǎn)品規(guī)格： 30 微升
應(yīng)用： IP

貨號: 26903

規(guī)格: 30 ?L

基因符號: RAC1

描述: Anti-Rac1-GTP Monoclonal Antibody

背景介紹: Small GTPases are a super-family of cellular signaling regulators. Rac belongs to the Rho sub-family of GTPases that regulate cell motility, cell division, and gene tranion. GTP binding increases the activity of Rac, and the hydrolysis of GTP to GDP renders it inactive. GTP hydrolysis is aided by GTPase activating proteins (GAPs), while exchange of GDP for GTP is facilitated by guanine nucleo exchange factors (GEFs).

原: Recombinant full length protein of active Rac1

經(jīng)過測試的應(yīng)用: IP, IHC, IF

推薦稀釋比: IP: 1 ?g for 1~2 mg total cellular proteins IHC, IF: 1:50-1:250

濃度: 1 mg/ml

宿主: Mouse

形式: Liquid

克隆性: Monoclonal

亞型: IgM

純度: Purified from ascites

防腐劑: No

成分: PBS (without Mg2+ and Ca2+), pH 7.4, 150 mM NaCl, 50% glycerol

種屬反應(yīng)性: Anti-Rac1-GTP monoclonal antibody recognizes active Rac1 from vertebrates.

儲存條件: Store at -20°C. oid repeated freezing and thawing

沉淀/蛋白質(zhì)印跡:

Rac Activation Assay. MEF cells were treated with (lane 2) or without (lane 1) PDGF. Cell lysates were incubated with an anti-Rac-GTP monoclonal antibody (Cat. # 26903) (top panel). Th precipitated active Rac was immunoblotted with an anti-Rac rit polyclonal antibody (Cat. # 21003). The bottom panel shows the Western blot with anti-Rac of the cell lysates used (5% of that used in the top panel).

熒光:

Immunofluorescence for the active Rac using Anti-Rac1-GTP Mouse Monoclonal Antibody [26903] shows Rac-GTP immunolabeling (green) in combination with cofilin (red) on brain tissue sections. The tissue sections were fixed with -20 ?C methanol or 4% paraformaldehyde (fixation time 1 hr) and stained with antibody at 1: 1000 in 0.1M Phosphate buffer with 0.3% Triton X, and 4% BSA for 24h at room temperature. Secondary antibodies were anti-mouse AlexaFluor488 and anti-rit AlexaFluor594 at 1: 1000.

標(biāo)簽： Rac- Rac acit Rac-GTP 小鼠單抗 Rac antibody acitve Rac抗體武漢市小鼠單抗費斯德武漢市小鼠單抗費斯德廠家