Gαi-GTP 小鼠單抗類目 > 商鋪首頁 > 產(chǎn)品展示 > 武漢市小鼠單抗廠家_Gαi-GTP 小鼠單抗/2022抗體現(xiàn)貨廠家/NewEast Biosciences總代理

Gαi-GTP 小鼠單抗/2022抗體現(xiàn)貨廠家/NewEast Biosciences總代理

[Gαi-GTP 鼠單抗

Gαi-GTP 活性單抗

active Gαi抗體

Gαi antibody]

武漢市小鼠單抗廠家

價格： 電議

物流： 湖北武漢市| 賣家支付運(yùn)費(fèi)

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[Gαi-GTP 鼠單抗 Gαi-GTP 活性單抗 active Gαi抗體 Gαi antibody]

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詳細(xì)信息（Gαi-GTP 小鼠單抗/2022抗體現(xiàn)貨廠家/NewEast Biosciences總代理）

分類： 抗體
級別： 其它
含量： 電議
產(chǎn)品規(guī)格： 30 微升
CAS： 無
應(yīng)用： IP
貨號： 26901
品牌： NewEast Biosciences

貨號: 26901

基因符號: Gnai

描述: Anti-Gαi-GTP Mouse Monoclonal Antibody

背景介紹: Heterotrimeric G proteins are critical cellular signal transducers. Gαi represents one sub-family of G proteins that could mediate the inhibition of adenylyl cyclases. Other biochemical and physiological functions of Gα i proteins are being explored.

原: Recombinant full length protein of active Gα i1

經(jīng)過測試的應(yīng)用: IP, IHC, IF

推薦稀釋比:
IP: 1 ?g for 1~2 mg total cellular proteins
IHC, IF: 1:50-1:250

濃度: 1 mg/ml

宿主: Mouse

形式: Liquid

克隆性: Monoclonal

亞型: IgG1

純度: Purified from ascites

防腐劑: No

成分: PBS (without Mg 2+ and Ca 2+ ), pH 7.4, 150 mM NaCl, 50% glycerol

種屬反應(yīng)性: Anti-active Gα i antibody recognizes active Gα i1, Gα i2, and Gα i3 of vertebrates

儲存條件: Store at -20°C. oid repeated freezing and thawing

沉淀/蛋白質(zhì)印跡:

Gαi activation assay.
A. CHO cells were transfected with wild-type Gα i1 (lanes 1 and 2) or constitutively active Gα i1-Q204L (lane 3). Cell lysates were treated with GDP(lane 1) or GTPγS (lane 3). Lysates were then incubated with an anti-active Gα i monoclonal antibody (Cat. No.26901) (top panel). The precipitated active Gα i was immunoblotted with an anti-Gα i monoclonal antibody (Cat. No. 26003). The bottom panel shows the Western blot with anti-Gα i monoclonal antibody (Cat.No. 26003) of the cell lysates.
B. HEK293 cells stably expressing human m2 mAChR were treated with (lane 2) or without (lane 1)carbachol. Cell lysates were then incubated with an anti-active Gα i monoclonal antibody (Cat. No. 26901) (top panel).The precipitated active Gα i was immunoblotted with an anti-Gα i rit polyclonal antibody (Cat. No. 21006). The bottom panel shows the Western blot with anti-tubulin of the cell lysates.

標(biāo)簽： Gαi- Gαi- acti Gαi Gαi-GTP 鼠單抗 Gαi-GTP 活性單抗 active Gαi抗體 Gαi antibody 武漢市小鼠單抗武漢市小鼠單抗廠家