[Anti-IDH2鼠抗 Anti-IDH2抗體 IDH2(R172W)]
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| 貨號: 26164 |
| 基因符號: IDH2(R172W) |
| 描述: Anti-IDH2(R172W) Mouse Monoclonal Antibody |
| 背景介紹: Isocitrate dehydrogenase (IDH) catalyze the oxidative decarboxylation of isocitrate to 2-oxoglutarate. The isocitrate and isopropylmalate dehydrogenases family has three members, IDH1, IDH2 and IDH3. IDH2 plays a role in intermediary metabolism and energy production. Defects in IDH2 are the cause of D-2-hydroxyglutaric aciduria type 2 (D2HGA2). Somatic mosaic mutations of this protein have also been found associated to Ollier disease and Maffucci syndrome, and R172W IDH2 mutations do exist in diffusely infiltrative gliomas. |
| 原: A synthetic pep from the internal region of IDH2 which includes the mutation of R172W, human origin |
| 經(jīng)過測試的應(yīng)用: ELISA, WB, IF, IHC |
| 推薦稀釋比: ELISA: 1: 1000-1: 5000 WB: 1: 100-1: 1000 IF: 1: 50-1: 100 IHC: 1: 50-1: 100 |
| 濃度: 1 mg/ml |
| 宿主: Mouse |
| 形式: Liquid |
| 克隆性: Monoclonal |
| 亞型: IgG |
| 純度: Purified from ascites |
| 防腐劑: No |
| 成分: PBS (without Mg2+ and Ca2+), pH 7.4, 150 mM NaCl, 50% glycerol |
| 種屬反應(yīng)性: recognizes IDH1(R172W) of vertebrates |
| 儲存條件: Store at -20°C. oid repeated freezing and thawing |
| 蛋白質(zhì)印跡: Western blot analysis of recombinant IDH2(R172W) and wildtype proteins. Purified His-tagged IDH2(R172W) (lane 2) and wildtype protein (lane 1) were blotted with anti IDH2(R172W) monoclonal antibody (Cat. #26164). 熒光: Immunofluorescence of cells expressing IDH2 proteins with anti IDH2(R172W) antibody. HEK293T cells were transfected with pCDNA3-GFP-IDH2 (WT) plasmid (left column) or pCDNA3-GFP-IDH2(R172W) plasmid (right column), then fixed and stained with anti-IDH2(R172W) monoclonal antibody (Cat. #26164).
武漢費斯德生物科技有限公司是美國NewEast Biosciences在中國的辦事處。NewEast Biosciences 在十二年前率先研發(fā)倆種獨特的抗體。這倆種抗體僅僅識別活性的GTP酶或者突變的Oncogene。 GTP酶涉及(1)響應(yīng)細(xì)胞表面受體的信號轉(zhuǎn)導(dǎo),包括跨膜受體,例如介導(dǎo)味覺、嗅覺和視覺的那些,(2)核糖體的蛋白質(zhì)生物合成,(3)調(diào)節(jié)細(xì)胞分化、增殖、分裂和運動,(4)蛋白質(zhì)通過膜的易位,(5)細(xì)胞內(nèi)囊泡的運輸,以及囊泡介導(dǎo)的分泌和攝取,通過GTP酶控制囊泡外殼組裝。Oncogene側(cè)是誘發(fā)的基因。 我公司將向你提供以下三種抗體或者試劑盒: (1) 僅識別 GTP酶的活性構(gòu)型的產(chǎn)品, 它可以讓你能夠量化GTP酶在細(xì)胞中的活性和分布。(2) 識別突變 Oncogene蛋白, 但不認(rèn)識相應(yīng)型的抗體。 (3) 對 cAMP 和 cGMP 具有超親和力(無需乙?;〦LISA檢測試劑盒。這些產(chǎn)品被將近一千篇同行評議的文章所引用。 2021年武漢費斯德生物科技有限公司和NewEast Biosciences了武漢紐斯特生物技術(shù)有限公司所有的產(chǎn)品和細(xì)胞株。因此, 紐斯特不再擁有這些產(chǎn)品銷售權(quán)。如果您在 2022 年 6 月 1 日之后從 紐斯特購買相同的產(chǎn)品, 你不應(yīng)該在你的中引用 紐斯特的產(chǎn)品為NewEast Biosciences產(chǎn)品。 ** 本公司銷售的所有產(chǎn)品僅供實驗科研使用,不用于人體及臨床診斷。 |
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