[Anti-BRaf鼠單抗 BRAF(V600D) BRAFV600D抗體]
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| 貨號: 26184 |
| 基因符號: BRAF, BRAF1, RAFB1 |
| 描述: Anti-BRaf(V600D) Mouse Monoclonal Antibody |
| 背景介紹: B-Raf is a member of the Raf family of Ser/Thr protein kinases. It functions downstream of Ras to regulate the MAP kinase signaling pathway. Mutations in the BRAF gene cause diseases. Inherited mutations in BRAF cause cardiofaciocutaneous syndrome. Acquired mutations in BRAF have been found in cancers. |
| 原: A synthetic pep from the internal region of BRaf(V600D), human origin |
| 經(jīng)過測試的應用: ELISA, WB, IF, IHC |
| 推薦稀釋比: ELISA 1: 1000-1: 5000 WB 1: 500-1: 2000 IF 1: 100-1: 200 IHC 1: 100-1: 200 |
| 濃度: 0.5 mg/ml |
| 宿主: Mouse |
| 形式: Liquid |
| 克隆性: Monoclonal |
| 亞型: IgG |
| 純度: Purified from ascites |
| 防腐劑: No |
| 成分: PBS (without Mg2+ and Ca2+), pH 7.4, 150 mM NaCl, 50% glycerol |
| 種屬反應性: Recognizes B-Raf V600D, but not wild-type B-Raf proteins from vertebrates. |
| 儲存條件: Store at -20°C. oid repeated freezing and thawing |
| 蛋白質(zhì)印跡: Western blot analysis of recombinant B-raf proteins. Purified His-tagged BRaf(V600D) protein (amino acids 513-693, lane 2) and corresponding wild type protein (lane 1) were blotted with Anti-BRaf(V600D) monoclonal antibody (Cat. # 26184). 熒光: Immunofluorescence of cells expressing B-Raf proteins with Anti-BRaf(V600D) antibody. HEK293T cells were transfected with pCDNA3-GFP-B-Raf (WT) plasmid (left column) or pCDNA3-GFP-BRaf(V600D) plasmid (right column), then fixed and stained with Anti-BRaf(V600D) monoclonal antibody (Cat. #26184). |
注冊資金:100萬-500萬
聯(lián)系人:付女士
固話:027-63497286
移動手機:18162422647
企業(yè)地址:湖北 武漢市 東西湖區(qū)