價(jià)格: 電議
物流: 湖北 武漢市| 賣家支付運(yùn)費(fèi)
可銷售總量: 99件
手機(jī): 18162422647 郵箱: nebio0923@163.com
傳真: 尚未完善 地址: 湖北 武漢市
[anti BRAF抗體 BRAF(V600A) BRAFV600A單抗]
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手機(jī):
| 貨號: 26199 |
| 基因符號: BRAF, BRAF1, RAFB1 |
| 描述: Anti-BRAF(V600A) Mouse Monoclonal Antibody |
| 背景介紹: BRAF belongs to a family of serine-threonine protein kinases. As a part of a signaling pathway known as the the RAS/MAPK pathway, it plays an important part in many cellular processes. The processes include cell proliferation, differentiation and transcriptional regulation. Mutations in the BRAF gene cause diseases. Inherited mutations in BRAF cause cardiofaciocutaneous syndrome. Acquired mutations in BRAF have been found in cancers. |
| 原: A synthetic pep from the internal region of BRAF, which includes the mutation of V600A, human origin |
| 經(jīng)過測試的應(yīng)用: ELISA, WB, IHC |
| 推薦稀釋比: ELISA: 1: 1000-1: 5000 WB: 1: 500-1: 1000 IF: 1: 50-1: 100 IHC: 1: 25-1: 100 |
| 濃度: 1 mg/ml |
| 宿主: Mouse |
| 形式: Liquid |
| 克隆性: Monoclonal |
| 亞型: IgG |
| 純度: Purified from ascites |
| 防腐劑: No |
| 成分: PBS (without Mg2+ and Ca2+), pH 7.4, 150 mM NaCl, 50% glycerol |
| 種屬反應(yīng)性: Recognizes V600A mutant, but not wild-type BRAF of vertebrates |
| 儲存條件: Store at -20°C. oid repeated freezing and thawing |
| 蛋白質(zhì)印跡: Western blot analysis of recombinant BRAF proteins. Purified His-tagged BRAF(V600A) protein (lane 1) , corresponding wildtype protein (lane 2) , His-tagged BRAF (V600R) protein (lane 3), His-tagged BRAF (V600M) protein (lane 4), His-tagged BRAF (V600K) protein (lane 5), His-tagged BRAF (V600G) protein (lane 6), His-tagged BRAF (V600D) protein (lane 7) and His-tagged BRAF (V600E) protein (lane 8) were blotted with Anti-BRAF(V600A) monoclonal antibody (Cat. #26199). Immunofluorescence of cells expressing BRAF proteins with Anti-BRAF(V600A) antibody. HEK293T cells were transfected with pCDNA3-GFP-BRAF (WT) plasmid, pCDNA3-GFP-BRAF(V600A) plasmid, pCDNA3-GFP-BRAF (V600D) plasmid, pCDNA3-GFP-BRAF (V600G) plasmid pCDNA3-GFP-BRAF (V600M) plasmid or pCDNA3-GFP-BRAF (V600E) plasmid, then fixed and stained with Anti-BRAF(V600A) monoclonal antibody (Cat. #26199). |
注冊資金:100萬-500萬
聯(lián)系人:付女士
固話:027-63497286
移動(dòng)手機(jī):18162422647
企業(yè)地址:湖北 武漢市 東西湖區(qū)