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BRAF(V600E) 小鼠單抗/Anti-BRaf(V600E) Mouse Monoclonal Antibody

[Anti-BRaf抗體

BRAF(V600E)

BRAF(V600E]

武漢市小鼠單抗廠家

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[Anti-BRaf抗體 BRAF(V600E) BRAF(V600E]

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詳細(xì)信息（BRAF(V600E) 小鼠單抗/Anti-BRaf(V600E) Mouse Monoclonal Antibody）

分類： 抗體
級(jí)別： 其它
含量： 電議
產(chǎn)品規(guī)格： 100 微升
應(yīng)用： ELISA WB IF IHC
貨號(hào)： 26039
品牌： NewEast Biosciences

貨號(hào): 26039

基因符號(hào): BRaf

描述: Anti-BRaf(V600E) Mouse Monoclonal Antibody

背景介紹: B-Raf is a member of the Raf family of Ser/Thr protein kinases. It functions downstream of Ras to regulate the MAP kinase signaling pathway. Mutations in the BRAF gene cause diseases. Inherited mutations in BRAF cause cardiofaciocutaneous syndrome. Acquired mutations in BRAF have been found in cancers

原: A synthetic pep from the internal region of BRaf(V600E), human origin

經(jīng)過測(cè)試的應(yīng)用: ELISA, WB, IHC, IP

推薦稀釋比:
ELISA: 1: 100-1: 5000
WB: 1: 100-1: 2000
IHC: 1: 100-1: 1000

濃度: 1 mg/ml

宿主: Mouse

形式: Liquid

克隆性: Monoclonal

亞型: IgG2b

純度: Purified from ascites

防腐劑: No

成分: PBS (without Mg2+ and Ca2+), pH 7.4, 150 mM NaCl, 50% glycerol

種屬反應(yīng)性: Recognizes B-Raf(V600E), but not wild type B-Raf proteins from vertebrates.

儲(chǔ)存條件: Store at -20°C. oid repeated freezing and thawing

蛋白質(zhì)印跡:

Western blot analysis of recombinant B-raf proteins with anti-BRaf(V600E) antibody.

Purified His – tagged B-Raf( V600E ) ?amino acids 513-693)(lane1) ? corresponding wild type protein(lane 2), His – tagged B-Raf( V600D ) (lane 3) , His – tagged B-Raf( V600A ) (lane 4) , His – tagged B-Raf( V600G ) (lane 5) , His – tagged B-Raf( V600K ) (lane 6), His – tagged B-Raf( V600R ) (lane 7), and His – tagged B-Raf( V600M ) (lane 8) were blotted with anti-B-Raf(V600E) monoclonal antibody (Cat. # 26039) .

熒光:

Immunofluorescence of cell expressing BRaf protein with anti-BRaf(V600E) antibody . HEK293T cells were transfected with pCDNA3-GFP- B-Raf( V600E )plasmid,pCDNA3-GFP- B-Raf( WT )plasmid, pCDNA3-GFP- B-Raf( V600K )plasmid,pCDNA3-GFP- B-Raf( V600R )plasmid, pCDNA3-GFP- B-Raf( V600A )plasmid,pCDNA3-GFP- B-Raf( V600D ) plasmid, pCDNA3-GFP-B-Raf(V600M)plasmid, or pCDNA3-GFP-B-Raf(V600G)plasmid, then fixed and stained with anti-B-Raf(V600E) monoclonal antibody (Cat. # 26039).

Immunohistochemistry:

B-Raf(V600E) monoclonal antibody (Cat. # 26039). Tissue samples were fixed with formaldehyde and blocked with 1% serum for 15 min at 37 °C. Antigen retrieval was by heat mediation in citrate buffer (pH6). Samples were then incubated with primary antibody (1: 100) overnight at 4°C. A HRP-conjugated Goat anti-mouse IgG (dilution 1: 50) was used as secondary antibody.

Immunohistochemical analysis of paraffin-embedded Thyroid Carcinoma tissue -with anti-BRaf (V600E) monoclonal antibody (Cat. # 26039). Tissue samples were fixed with paraffin. Samples were then incubated with primary antibody (1: 100) overnight at 4°C. An HRP-conjugated Goat anti-mouse IgG (dilution 1: 50) was used as the secondary antibody. Allele-specific PCR validated to be Negative for BRAF V600E.

圖片.png

Immunohistochemical analysis of paraffin-embedded Thyroid Carcinoma tissue -with anti BRaf(V600E) monoclonal antibody (Cat. # 26039). Tissue samples were fixed with paraffin. Samples were then incubated with primary antibody (1: 100) overnight at 4°C.A HRP-conjugated Goat anti-mouse IgG (dilution 1: 50) was used as secondary antibody.Allele specific PCR validated to be Negative for BRAF V600E.

標(biāo)簽： Anti BRAF BRAF Anti-BRaf抗體 BRAF(V600E) BRAF(V600E 武漢市小鼠單抗武漢市小鼠單抗廠家